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fastpure cell tissue total rna isolation kit v2  (Vazyme Biotech Co)


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    Vazyme Biotech Co fastpure cell tissue total rna isolation kit v2
    Fastpure Cell Tissue Total Rna Isolation Kit V2, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 3545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isolation+kit/FastPure+Cell+Tissue+Total+RNA+Isolation+Kit+V2/pmc13085024-88-10-18
    Average 99 stars, based on 3545 article reviews
    fastpure cell tissue total rna isolation kit v2 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Electroacupuncture combined with rTMS promotes neuronal regeneration via DNMT1-mediated PI3K-AKT pathway in cerebral palsy model
    Article Snippet: .. Total RNA was extracted with an isolation kit (RC112; Vazyme, Nanjing, China). .. RNA quality was verified (A260/A280 >1.8) using a spectrophotometer (Jiapeng). cDNA was synthesized from 500 ng RNA using HiScript III cDNA Synthesis Kit (R312; Vazyme, Nanjing, China). qPCR was performed using SYBR Master Mix (Q712; Vazyme) on a CFX96 system (Bio-Rad, Hercules, CA, USA).

    Article Title: The miR395b-ABI5 module regulates amylopectin branching and biosynthesis and affects lotus root quality.
    Article Snippet: The miRNA was extracted from the lotus roots using the miRNA Extraction and Isolation Kit (TIANGEN, Beijing, China). cDNA was synthesized using the miR395b stem-loop primer and SuperScript III RT-PCR (Vazyme, Nanjing, China), and the lotus U6 of lotus was used as the internal reference gene of miRNA, and the expression of mature miR395b was detected by using the miRNA-specific forward primer, generalized reverse primer, and stem-loop RT-qPCR. .. Total RNA was extracted from lotus roots petals by a Isolation Kit (Vazyme, RC411-01) (Wang et al. 2020). .. Reverse transcription was performed with 1 μg total RNA using HiScript II Q Select RT SuperMix kit (Vazyme, Nanjing, China). qPCR was performed on a CFX connect Real-Time System (Bio-Rad, Shanghai, China) and a ChamQ SYBR qPCR Master Mix was used to conduct qPCR experiments (Vazyme, Nanjing, China).

    Article Title: MicroRNA-124 attenuates PTSD-like behaviors and reduces the level of inflammatory cytokines by downregulating the expression of TRAF6 in the hippocampus of rats following single-prolonged stress.
    Article Snippet: .. An isolation kit (Vazyme Biotech, Nanjing, China) was used to extract total RNA from rat hippocampus. .. The miRNA first strand cDNA synthesis kit (Vazyme Biotech) was used to reversely transcribe the extracted RNA into cDNA.

    Article Title: The miR395b–ABI5 module regulates amylopectin branching and biosynthesis and affects lotus root quality
    Article Snippet: The miRNA was extracted from the lotus roots using the miRNA Extraction and Isolation Kit (TIANGEN, Beijing, China). cDNA was synthesized using the miR395b stem-loop primer and SuperScript III RT-PCR (Vazyme, Nanjing, China), and the lotus U6 of lotus was used as the internal reference gene of miRNA, and the expression of mature miR395b was detected by using the miRNA-specific forward primer, generalized reverse primer, and stem-loop RT-qPCR. .. Total RNA was extracted from lotus roots petals by a Isolation Kit (Vazyme, RC411-01) ( ). .. Reverse transcription was performed with 1 μ g total RNA using HiScript II Q Select RT SuperMix kit (Vazyme, Nanjing, China). qPCR was performed on a CFX connect Real-Time System (Bio-Rad, Shanghai, China) and a ChamQ SYBR qPCR Master Mix was used to conduct qPCR experiments (Vazyme, Nanjing, China).

    Article Title: Artesunate Nanoplatform Targets the Serine–MAPK Axis in Cancer‐Associated Fibroblasts to Reverse Photothermal Resistance in Triple‐Negative Breast Cancer
    Article Snippet: .. Total RNA was collected via an isolation kit (Vazyme, RC11201) and transcribed to cDNA via PrimeScript (Vazyme, R323) according to the manufacturer's protocol. qPCR was performed on a real‐time qPCR instrument (Applied Biosystems, QuantStudio 7 Flex) using ChamQ Universal SYBR qPCR Master Mix (Vazyme, Q711‐02). β‐Actin was selected as an internal reference gene for normalization, and the data were analyzed via the comparative CT method. ..

    Real-time Polymerase Chain Reaction:

    Article Title: MicroRNA-124 Attenuates PTSD-like Behaviors and Reduces the Level of Inflammatory Cytokines by Downregulating the Expression of TRAF6 in the Hippocampus of Rats Following Single-prolonged Stress
    Article Snippet: All grids were detected under a transmission electron microscope (H-7650, Hitachi Limited, Japan) operating at 80 kV. .. Page 8/32 Real-time quantitative PCR (RT-qPCR) The isolation kit (Vazyme Biotech, Nanjing, China) was used to extract total RNA from rat hippocampus and the miRNA rst strand cDNA synthesis kit (Vazyme Biotech) was used to reversely transcribe the extracted RNA into cDNA. .. Real time PCR reaction were performed with speci c primers (GenePharma, Shanghai, China) using the StepOnePlus system (Applied Biosystems, USA).

    Article Title: Artesunate Nanoplatform Targets the Serine–MAPK Axis in Cancer‐Associated Fibroblasts to Reverse Photothermal Resistance in Triple‐Negative Breast Cancer
    Article Snippet: .. Total RNA was collected via an isolation kit (Vazyme, RC11201) and transcribed to cDNA via PrimeScript (Vazyme, R323) according to the manufacturer's protocol. qPCR was performed on a real‐time qPCR instrument (Applied Biosystems, QuantStudio 7 Flex) using ChamQ Universal SYBR qPCR Master Mix (Vazyme, Q711‐02). β‐Actin was selected as an internal reference gene for normalization, and the data were analyzed via the comparative CT method. ..

    cDNA Synthesis:

    Article Title: MicroRNA-124 Attenuates PTSD-like Behaviors and Reduces the Level of Inflammatory Cytokines by Downregulating the Expression of TRAF6 in the Hippocampus of Rats Following Single-prolonged Stress
    Article Snippet: All grids were detected under a transmission electron microscope (H-7650, Hitachi Limited, Japan) operating at 80 kV. .. Page 8/32 Real-time quantitative PCR (RT-qPCR) The isolation kit (Vazyme Biotech, Nanjing, China) was used to extract total RNA from rat hippocampus and the miRNA rst strand cDNA synthesis kit (Vazyme Biotech) was used to reversely transcribe the extracted RNA into cDNA. .. Real time PCR reaction were performed with speci c primers (GenePharma, Shanghai, China) using the StepOnePlus system (Applied Biosystems, USA).



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